Delhi: June 11:The Indian Council of Medical Research (ICMR) has recommended only reverse transcription polymerase chain reaction (RT-qPCR) test for novel coronavirus testing. Researchers at the Centre for Cellular and Molecular Biology (CCMB) have developed a new low-cost and low-tech required test for SARS-CoV-2 testing. This test is known as reverse transcription nested PCR (RT-nPCR) test.
This test does not require real-time quantitative RT-qPCR. The RT-nPCR developed by the CCMB research team has shown comparable performance to the standard RT-qPCR test. The nested PCR (RT-nPCR) approach does not depend on RT-qPCR but uses standard RT-PCR as part of an endpoint assay.
